Cystatin C ELISA Kit (Monkey) : 96 Wells (OKIA00184)

Brand

AVIVA Systems Biology

short description

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the CYS present in samples reacts with the anti-CYS antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-CYS, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromoﾬgenic substrate, 3,3ﾒ,5,5ﾒ-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of CYS in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of CYS in the test sample. The quantity of CYS in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Immunoperoxidase Assay for Determination of Cystatin C in Monkey Samples

spezie

Monkey

Predicted Homology Based on Immunogen Sequence:

Monkey

detection target

Cystatin C (Monkey)
description of target: Monkey Cystatin C1
gen symbol: CST3
Gene ID: 574313 ID:O19092

alias

Cystatin-3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin

sensitivity

0.3303 ng/ml

pack size

96 Wells

reconstitution and storage

1. DILUENT The 1X Diluent Concentrate is stable until the expiraﾬtion date. It should be stored at 4-8?C. 2. WASH SOLUTION The 20X Wash Solution Concentrate is stable until the expiraﾬtion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25?C) or at 4-8?C. 3. DETECTION ANTIBODY 100X Undiluted Biotin conjugated anti-CYS should be stored at 4-8?C and diluted immediately prior to use. The working conjugate solution is stable for up to 1 hour when stored in the dark. 4. HRP-STREPTAVIDIN 100X Undiluted horseradish peroxidase conjugated streptavidin should be stored at 4-8?C and diluted immediately prior to use. The working conjugate solution is stable for up to 1 hour when stored in the dark. 5. CHROMOGEN-SUBSTRATE SOLUTION The Substrate Solution should be stored at 4-8?C and is stable until the expiration date. 6. STOP SOLUTION The Stop Solution should be stored at 4-8?C and is stable until the expiration date. 7. ANTI-MONKEY CYS ELISA MICRO PLATE Anti-Monkey CYS coated wells are stable until the expiration date, and should be stored at 4-8?C in sealed foil pouch with desiccant pack. 8. MONKEY CYS CALIBRATOR Long Term Storage: Upon receipt, aliquot the calibrator and store them frozen. They will be stable until expiration date. Short Term Storage: the calibrator is stable for up to 14 days at 4ﾺC. The working standard solutions should be prepared immediately prior to use. INDICATIONS OF INSTABILITY If the test is performing correctly, the results observed with the standard solutions should be within 20 % of the expected values.