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Cloud-Clone
Chemiluminescent immunoassay for Antigen Detection.Research focus: Tumor immunity; Infection immunity; Cardiovascular biology; Rheumatology; ;
Ovis aries; Ovine (Sheep)
This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 1 (MMP1).
No significant cross-reactivity or interference between Matrix Metalloproteinase 1 (MMP1) and analogues was observed.
Matrix Metalloproteinase 1
CLGN; CLG1; Collagenase; Interstitial Collagenase; Vertebrate Collagenase; Fibroblast Collagenase
The minimum detectable dose of this kit is typically less than 6.35 pg/mL
156.25-10000 pg/mL
ISO 9001:2008, ISO 13485:2003
Serum, plasma and other biological fluids
48T, 96T, 5x96T, 10x96T
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 1 (MMP1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 1 (MMP1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
2h, 40min
All the kits should stored according to the manual. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree upon receipt while the others should be at 4 degree.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Double-antibody SandwichTest principle: The microplate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 1 (MMP1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 1 (MMP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Matrix Metalloproteinase 1 (MMP1) level in the sample or standard.;
Pre-coated, ready to use 96-well strip plate: 1Plate sealer for 96 wells: 4Standard: 2 Standard Diluent: 1x20mL Detection Reagent A: 1x120µ L Assay Diluent A: 1x12mL Detection Reagent B: 1x120µ L Assay Diluent B: 1x12mL TMB Substrate: 1x9mL Stop Solution: 1x6mL Wash Buffer (30 x concentrate): 1x20mL Instruction manual: 1
1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 100µ L Substrate Solution. Incubate 10 minutes at 37° C;
8. Read RLU value immediately.
Hersteller | Cloud-Clone |
---|---|
Herstellerland | China |
Sensitivity | The minimum detectable dose of this kit is typically less than 6.35 pg/mL |
Specific Against | Sheep |
Antigen Name | CLIA Kit for Matrix Metalloproteinase 1 (MMP1) |
Detection Range | 156.25-10000 pg/mL |
Zertifikat | ISO 9001:2008, ISO 13485:2003 |
Alias | CLGN/ CLG1/ Collagenase/ Interstitial Collagenase/ Vertebrate Collagenase/ Fibroblast Collagenase |