Chlamydia trachomatis IgA

Brand

GENWAY

Overview

Intended Use: The Chlamydia trachomatis IgA-ELISA is intended for the qualitative determination of IgA class antibodies against Chlamydia trachomatis in human serum or plasma (citrate). Introduction: Chlamydiae are nonmotile Gram negative and obligatory intracellular growing bacteria which form characteristic inclusions within the cytoplasm of parasitized cells. They are easily visible in the light microscope. Three different Chlamydia species pathogenic for humans are known: Chlamydia trachomatis Chlamydia pneumoniae and Chlamydia psittaci and one species only pathogenic for animals (C. pecorum). Chlamydia trachomatis is the most prevalent agent of sexually transmitted diseases worldwide (400-500 million cases) and the number of infections is constantly growing during childbirth causing conjunctivitis or pneumonia in newborns. Untreated cases of chlamydial infection can lead to chronic salpingitis possibly resulting in ectopic pregnancy or infertility. In males C. trachomatis is a major cause of non-gonococcal urethritis. A severe problem in Chlamydia infections is the frequent asymptomatic insidious course which may result in the initiation of chronic diseases. In many instances primary infections are not recognized and only the sequelae caused by ascended persisting agents are diagnosed. Infection may be identified byMicroscopy: Giemsa stainPCRSerology: Detection of antigens by ELISA Detection of antibodies by IF EIA ELISAPrinciples of the assay: The qualitative immunoenzymatic determination of IgA-class antibodies against Chlamydia trachomatis is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells are precoated with Chlamydia trachomatis antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgA conjugate is added. This conjugate binds to the captured Chlamydia-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Chlamydia-specific IgA antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader. Storage and Stability: The reagents are stable up to the expiry date stated on the label when stored at 2. . . 8 °C. Limitations of the Test: Bacterial contamination or repeated freeze-thaw cycles of the specimen may affect the absorbance values. Diagnosis of an infectious disease should not be established on the basis of a single test result. A precise diagnosis should take into consideration clinical history symptomatology as well as serological data. In immunocompromised patients and newborns serological data only have restricted value. The Chlamydia trachomatis antigen which is coated on the plates is comprised of elementary bodies. A cross reaction with Chlamydia pneumoniae cannot be excluded with sera containing antibodies to LPS and MOMP. References Hoyme U. B. Spitzbart H. (1996). Past and current prevalence of Chlamydia trachomatis in women in Germany. In: Chlamydia Research. Angelika Stary (ed. ). Proceedings of the third meeting of the European Society for Chlamydia Research Vienna Austria 11. -14. Sept. p. 391. Paavonen J. (1996). Chlamydia trachomatis: A major cause of mucopurulent cervicitis and pelvic inflammatory disease in women. In: Sexually Transmitted Diseases. Advances in Diagnosis amd Treatment. Curr. Probl. Dermatol. Elsner P. Eichmann A. (eds. ) Basel Karger Vol. 24 pp. 110-122. Petersen E. E. Clad A. (1995). Genitale Chlamydieninfektionen. Deutsches Ärzteblatt 92 Heft 5 A-277-282. Weström L. (1996). Consequences of genital Chlamydia infections in women. In: Chlamydia Research. Angelika Stary (ed. ). Proceedings of the third meeting of the European Society for Chlamydia Research Vienna Austria 11. -14. Sept. pp. 137-140. Weström L. V. (1996). Chlamydia and its effect on reproduction. J. Brit. Fertil. Soc. 1: 23-30.

spezie

other

detection target

Chlamydia trachomatis IgA

pack size

1x96 assay