BioAim Scientific (Kanada)
The Bioaim Canine TIMP-2 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of TIMP-2 in canine serum, plasma and cell culture media. An antibody specific for canine TIMP-2 was coated on a 96-well plate. Standards and samples are added to the wells and any TIMP-2 present binds to the immobilized antibody. The wells are washed and biotinylated anti-canine TIMP-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of TIMP-2 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Angiogenesis; Cancer Biomarker;
TIMP metallopeptidase inhibitor 2 ( TIMP-2 ) Background: TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14. TIMP-2 functions as both an MMP inhibitor and an activator. TIMPs inhibit all active MMPs, but different TIMPs inhibit different MMPs better than other TIMPs. For example, TIMP-1 inhibits MMP-7, MMP-9, MMP-1 and MMP-3 better than TIMP-2, and TIMP-2 inhibits MMP-2 more effectively than other TIMPs. In melanocytic cells TIMP2 gene expression may be regulated by MITF.
Serum, plasma, Cell culture supernatant
|Antigen Name||TIMP metallopeptidase inhibitor 2|
|Detection Range||20.5-5000 pg/ml|