Brucella IgM

Brand

GENWAY

Overview

Intended Use: The Brucella IgM-ELISA is intended for the qualitative determination of IgM class antibodies against Brucella in human serum or plasma (citrate). Introduction: Brucella is a small Gram-negative bacterium (0. 4-0. 8 μm in diameter and 0. 4-3. 0 μm in length) which is non-flagellated and non-spore-forming. They are named after the military doctor David Bruce who on Malta in 1887 isolated the pathogens from the spleen of a soldier who died of undulating fever. Four species are pathogenic to human: Brucella abortus Brucella melitensis Brucella suis and Brucella canis. All four species are exciters of Brucellosis a disease characterized by undulating fever. Depending on exciter the disease is also called Morbus Bang (B. abortus) or Malta fever (B. melitensis). The pathogens are transmitted from animals which are mainly affected. The infection is caused by contact with ill animals or their excrements as well as by non-pasteurized milk and milk products like fresh cheese from sheep or goat. Main entrances are skin wounds conjunctives and digestive tract. The intact pathogens are transported by granulocytes into local lymph nodes from where they spread haematogenous. All kind of organs can be infected. Symptoms depend up on the infected organ where an inflammation takes place. Until now the pathogenic mechanism is not completely understood. Brucellosis appears worldwide. In non-pasteurized milk and milk products Brucella is viably and infectious for weeks. Bovine brucellosis caused by Brucella abortus is still the most widespread form although reported incidence and prevalence of the disease vary widely from country to country (from 200 per 100 000 population). Brucella melitensis is endemic in areas where keeping of sheeps and goats is frequent. It causes serious human infections. Working with these bacteria in laboratories necessitates highest carefulness because of high contagiosity. Risk groups include abattoir workers meat inspectors animal handlers veterinarians and laboratorians. Brucellosis is a nationally notifiable disease and reportable to the local health authority. The presence of infection may be identified byHistology: Brucella species may be speciated by susceptibility to the dyes fuchsin and thioninSerology: Detection of antibodies by ELISAPrinciples of the assay: The qualitative immunoenzymatic determination of IgM-class antibodies against Brucella is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells are precoated with Brucella antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgM conjugate is added. This conjugate binds to the captured Brucella-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Brucella specific IgM antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader. Storage and Stability: The reagents are stable up to the expiry date stated on the label when stored at 2. . . 8 °C. Limitations of the Test: Bacterial contamination or repeated freeze-thaw cycles of the specimen may affect the absorbance values. Diagnosis of an infectious disease should not be established on the basis of a single test result. A precise diagnosis should take into consideration clinical history symptomatology as well as serological data. In immunocompromised patients and newborns serological data only have restricted value. References SALATA R. A. RAVDIN J. I. (1985) BRUCELLA SPECIES (BRUCELLOSIS). IN: MANDEL GL DOUGLAS RG BENNET JE EDS. PRINCIPLES AND PRACTICE OF INFECTIOUS DISEASES. NEW YORK: JOHN WILEY 1283-90ARIZA J. PELLICER T. PALLARÉS R. FOZ A. AND GUDIOL F. (1992) SPECIFIC ANTIBODY PROFILE IN HUMAN BRUCELLOSIS. CLIN. INF. DIS. 14 131-140BROUQUI P. AND RAOULT D. (2001) ENDOCARDITIS DUE TO RARE AND FASTIDIOUS BACTERIA. CLIN. MICROBIOL. REV. 14 177 -207GAD EL RAB M. O. AND KAMBAL A. M. (1998) EVALUATION OF A BRUCELLA ENZYME IMMUNOASSAY TEST (ELISA) IN COMPARISON WITH BACTERIOLOGICAL CULTURE AND AGGLUTINATION. J. INFECT. 36 197-201CORBEL M. J. (1997) BRUCELLOSIS: AN OVERVIEW. EMERG. INFECT. DIS. 3 213-221ZIMMERLI S. UND KÖPPEL C. (2005) BRUCELLOSE. BVET-MAGAZIN 3 36-37

spezie

other

detection target

Brucella IgM

pack size

1x96 assay