Bovine IgM

Brand

GENWAY

Overview

Intended Use: The total Bovine IgM test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgM in bovine biological samples. Range of Standard Curve 400 ng/ml - 12. 5 ng/ml Total Assay Time Total Incubation Time: 60 minutes PRINCIPLE OF THE ASSAY The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the IgM present in samples reacts with the anti-IgM antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing anti-IgM antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound IgM. Following another washing step the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate 3 3’ 5 5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgM in the sample tested; thus the absorbance at 450 nm is a measure of the concentration of IgM in the test sample. The quantity of IgM in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.

spezie

Bovine

detection target

IgM

pack size

1x96 Wells