Bovine/Goat/Sheep Heat Shock Protein 70 (HSP70) ELISA Kit

Brand

Biomatik

short description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 70 (HSP70). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein 70 (HSP70). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 70 (HSP70), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein 70 (HSP70) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research area:Tumor immunity; Infection immunity; Developmental science;

spezie

Bos taurus; Bovine (Cattle), Capra hircus; Caprine (Goat), Ovis aries; Ovine (Sheep)

Cross Reaction

This assay has high sensitivity and excellent specificity for detection of Heat Shock Protein 70 (HSP70). No significant cross-reactivity or interference between Heat Shock Protein 70 (HSP70) and analogues was observed.

detection target

Heat Shock Protein 70

alias

DnaK; 70 Kilodalton Heat Shock Proteins

sensitivity

1.27 ng/mL

detection range

3.12-200 ng/mL

certificate

The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

pack size

1x96, 5x96 and 10x96 Wells

Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock Protein 70 (HSP70) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock Protein 70 (HSP70) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

Assay time

3 hours

storage and Stability:

Storage: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Expiry Date: 8 months
Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Shipping Condition: Blue ice

Principle of the Elisa

Sandwich
Detection Method: Colorimetric

Note

For research use only. Not for diagnostic procedures.
Precaution of Use: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.