Adenovirus IgM -

Brand

GENWAY

Overview

Intended Use: The Adenovirus IgM ELISA is intended for the qualitative determination of IgM class antibodies against Adenovirus in human serum or plasma (citrate). Introduction: Adenoviruses are double-stranded DNA viruses of about 70-90 nm lacking an envelope. The capsid contains 252 capsomeres and shows icosahedral symmetry. The capsomeres consist of hexons pentons and fiberprotein trimers which are responsible for the induction of group- and type-specific antibodies. For the first time adenoviruses were isolated in 1953 from tonsils and adenoid tissue by Rowe. More than 80 adenoviruses are known at present. 47 out of them are pathogenic for men. They cause several diseases of different organic systems mainly eyes pharynx respiratory and gastrointestinal system. Adenovirus infections are common and frequent. Most infections appear during childhood. They pass of latently so that the virus can still be detected in tonsils after two years. It is excrete via saliva and faeces. Gate to body are mouth nasal pharynx and conjunctiva of the eye. Most infections pass off without symptoms. Around 5 % of all coughs and sneezes of children are caused by adenoviruses. Epidemics may occur in populations crowded together for example acute respiratory disease in military groups pharyngoconjunctival fever in swimming pools and epidemic keratoconjunctivitis in medical facilities. Infection of hospitals and swimming pools gratify special demands on hygienics. The presence of virus resp. infection may be identified by:Cell culture: cytopathogenic effect (CPE)PCRSerology: complement fixation (CF) neutralization (N) and hemagglutination-inhibition (HAI); Detection of antibodies and the hexon antigen by ELISA. Principles of the assay: The qualitative immunoenzymatic determination of IgM-class antibodies against Adenovirus is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells are precoated with Adenovirus antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgM conjugate is added. This conjugate binds to the captured Adenovirus-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Adenovirus specific IgM antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader. Storage and Stability: The reagents are stable up to the expiry date stated on the label when stored at 2. . . 8 °C. Limitations of the Test: Bacterial contamination or repeated freeze-thaw cycles of the specimen may affect the absorbance values. Diagnosis of an infectious disease should not be established on the basis of a single test result. A precise diagnosis should take into consideration clinical history symptomatology as well as serological data. In immunocompromised patients and newborns serological data only have restricted value. References Adrian T. J. Sassinek R. Wiegand:Genome type analysis of 480 isolates of adenovirus type 1 2 and 5. Arch Virol. 112 (1990) 235-248Donelli G. F. Superti A. Tinari et al. : Viral childhood diarrhoe in Rome: a diagnostic and epidemiological study. Microbiologica 16 (1993) 215-225Hierholzer J. C. : Adenovirus in the immunocompromised host. Clin. Microbiol. Rev. 5 (1992) 262-274Schmitz H. R. Wiegand W. Heinrich: Worlwide epidemiology of human adenovirus infectionsw. Am. J. Epid. 117 (1983) 455-466Wiegand R. :Adenovirus. In:Brandis H. W. Köhler H. J. Eggers G. Pulverer (ed. ):Med. Mikrobiologie 1994. Gustav Fischer Verlag Stuttgart Jena New York (1994) 767-769

spezie

other

detection target

Adenovirus IgM

pack size

1x96 assay