5-HIAA Kit

Brand

GENWAY

Overview

Intended Use: Enzyme immunoassay for the quantitative determination of 5-HIAA in human urine. Principle of the test: The assay procedure follows the basic principle of competitive ELISA whereby there is competition between a biotinylated and a non-biotinylated antigen for a fixed number of antibody binding sites. The amount of biotinylated antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium the free biotinylated antigen is removed by a washing step and the antibody bound biotinylated antigen is determined by use of anti-biotin alkaline phosphatase as marker and p-nitrophenyl phosphate as substrate. Quantification of unknowns is achieved by comparing the enzymatic activity of unknowns with a response curve prepared by using known standards. Summary and Explanation of Test: Storage and Stability: The kit is shipped at ambient temperature and should be stored at 2-8C. Keep away from heat or direct sun light. The storage and stability of specimen and prepared reagents is stated in the corresponding chapters. The microtiter strips are stable up to the expiry date of the kit in the broken but tightly closed bag when stored at 2-8C. Storage and Stability: The kit is shipped at ambient temperature and should be stored at 2-8C. Keep away from heat or direct sun light. The storage and stability of specimen and prepared reagents is stated in the corresponding chapters. The microtiter strips are stable up to the expiry date of the kit in the broken but tightly closed bag when stored at 2-8C. Limitation of the Test: Specimen collection has a significant effect on the test results. See SPECIMEN COLLECTION AND STORAGE for details.

spezie

other

detection target

5-HIAA

pack size

1x96 Wells