Sensitivity: The minimum detectable dose of Rat PCSK9 was determined to be approximately 47 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. Detection: 47 pg/ml
Sensitivity: The minimum detectable dose of Rhesus CD32a / Fc gamma RIIA / FCGR2A was determined to be approximately 7.8 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. Detection: 7.8 pg/ml
Sensitivity: The minimum detectable dose of HIV-1 gp120 / Glycoprotein 120 was determined to be approximately 39.06 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. Detection: 39.06 pg/ml
Sensitivity: The minimum detectable dose of Human HER2 / ErbB2 / CD340 was determined to be approximately 24 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. Detection: 24 pg/ml
Sensitivity: The minimum detectable dose of Rhesus CD16 / FCGR3 was determined to be approximately 12.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. Detection: 12.5 pg/ml
Sensitivity: The minimum detectable dose of Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin / HA was determined to be approximately 62.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. Detection: 62.5 pg/ml
Since 1971 ELISA is used for detection and or determining the concentration of proteins within analyte mixture, low molecular weight compounds (e.g. Toxins, hormones) or viruses.
The ELISA is based on an antibody-antigen detection. Basis of this is the immobilization of an antigen or antibody on a multiwell plate (e.g. Polystyrene). The analyte is bound in the subsequent step.
The antigens are bound by the antibodies via hydrogen bonds, ionic and hydrophobic interactions, and Van-der-Waals forces. This binding occurs on a key-lock principle and is very specific. The antibodies used can thereby be monoclonal (mAb) or polyclonal (pAb). Monoclonal antibodies are made from hybridoma cells, a fusion of a cancer cell (myeloma) and an antibody-producing cell (B-cell) which selectively bind a specific epitope on the antigen. Polyclonal antibodies are a mixture of different antibodies which recognize different epitopes on the surface of one antigen. Once successfully attached the detection is normally made by colorimetry.
The successful antigen-antibody binding and addition of a substrate leads to a color change in the microtiter plate, which is catalyzed by an enzyme that has been previously conjugated to the antibody or antigen.
For further information, please refer to our Data Library at any time.