Enterohemorrhagic E. coli (EHEC) STX2B / Syntaxin 2 ELISA Pair Set

Brand

Sino Biological

short description

The Enterohemorrhagic E. coli (EHEC) STX2B ELISA Pair Set is for the quantitative determination of Enterohemorrhagic E. coli (EHEC) STX2B. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for STX2B coated on a 96-well plate. Standards and samples are added to the wells, and any STX2B present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-STX2B monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of STX2B present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

spezie

E. coli

detection target

Shiga toxin II subunit B
Standard (antigene): Each vial contains 19 ng of recombinant E. coli STX2B. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 4 ng/mL is recommended.
capture antibody type: 1.0 mg/mL of mouse anti-E. coli STX2B monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 µg/mL in CBS before coating. (Catalog: # 40019-MM08)
detection antibody type: 0.25 mg/mL mouse anti-E. coli STX2B monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % glycerol, pH 7.4). Dilute to working concentration of 0.5 µg/mL in detection antibody dilution buffer before use. (Catalog: # 40019-MM13)
Lable: HRP

sensitivity

The minimum detectable dose of Enterohemorrhagic E. coli (EHEC) STX2B was determined to be approximately 62.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

Detection limitation

62.5 pg/ml

certificate

ISO9001-2008

pack size

5 and 15 Plates

storage and Stability

Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20°C to -80°C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.