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Elisa
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Sensitivity: The minimum detectable dose of HIV-1 p24 was determined to be approximately 15.6 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Detection: 15.6 pg/ml
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Sensitivity: The minimum detectable dose of Human IL12B / IL-12B was determined to be approximately 15.625 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Detection: 15.625 pg/ml
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Sensitivity: The minimum detectable dose of Human Interleukin-32 / IL-32 was determined to be approximately 23.4375 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Detection: 23.4375 pg/ml
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Sensitivity: The minimum detectable dose of Human IFN gamma / IFNG was determined to be approximately 78.125 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Detection: 78.125 pg/ml
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Sensitivity: The minimum detectable dose of Human TREM-2 / TREM2 was determined to be approximately 125 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Detection: 125 pg/ml
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Sensitivity: The minimum detectable dose of H7N9 ( A/Shanghai/1/2013 ) HA was determined to be approximately 62.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Detection: 62.5 pg/ml
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Sensitivity: The minimum detectable dose of Mouse CD5L was determined to be approximately 6.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Detection: 6.25 pg/ml
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Sensitivity: The minimum detectable dose of Cynomolgus CD200R was determined to be approximately 62.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Detection: 62.5 pg/ml
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Sensitivity: The minimum detectable dose of H3N2 (A/Brisbane/10/2007) HA was determined to be approximately 625 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Detection: 625 pg/ml
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THE ELISA
Since 1971 ELISA is used for detection and or determining the concentration of proteins within analyte mixture, low molecular weight compounds (e.g. Toxins, hormones) or viruses.
The ELISA is based on an antibody-antigen detection. Basis of this is the immobilization of an antigen or antibody on a multiwell plate (e.g. Polystyrene). The analyte is bound in the subsequent step.
The antigens are bound by the antibodies via hydrogen bonds, ionic and hydrophobic interactions, and Van-der-Waals forces. This binding occurs on a key-lock principle and is very specific. The antibodies used can thereby be monoclonal (mAb) or polyclonal (pAb). Monoclonal antibodies are made from hybridoma cells, a fusion of a cancer cell (myeloma) and an antibody-producing cell (B-cell) which selectively bind a specific epitope on the antigen. Polyclonal antibodies are a mixture of different antibodies which recognize different epitopes on the surface of one antigen. Once successfully attached the detection is normally made by colorimetry.
The successful antigen-antibody binding and addition of a substrate leads to a color change in the microtiter plate, which is catalyzed by an enzyme that has been previously conjugated to the antibody or antigen.
For further information, please refer to our Data Library at any time.