Sensitivity: The minimum detectable dose of Mouse TNFR2 / CD120b / TNFRSF1B was determined to be approximately 9.4 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. Detection: 9.4 pg/ml
Sensitivity: The minimum detectable dose of Mouse IL-1R1 / CD121a was determined to be approximately 62.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. Detection: 62.5 pg/ml
Sensitivity: The minimum detectable dose of Mouse CD8a was determined to be approximately 46.9 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. Detection: 46.9 pg/ml
Sensitivity: The minimum detectable dose of Mouse Coagulation Factor VII / FVII was determined to be approximately 24 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. Detection: 24 pg/ml
Sensitivity: The minimum detectable dose of Mouse TIM-1 / KIM-1 / HACVR was determined to be approximately 18.75 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. Detection: 18.75 pg/ml
Sensitivity: The minimum detectable dose of Mouse EpCAM / TROP-1 / TACSTD1 was determined to be approximately 78 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. Detection: 78 pg/ml
Sensitivity: The minimum detectable dose of Mouse CD54 / ICAM1 was determined to be approximately 6.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. Detection: 6.25 pg/ml
Sensitivity: The minimum detectable dose of Mouse Endoglin / CD105 / ENG was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. Detection: 31.25 pg/ml
Sensitivity: The minimum detectable dose of Mouse CD14 was determined to be approximately 78.1 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. Detection: 78.1 pg/ml
Since 1971 ELISA is used for detection and or determining the concentration of proteins within analyte mixture, low molecular weight compounds (e.g. Toxins, hormones) or viruses.
The ELISA is based on an antibody-antigen detection. Basis of this is the immobilization of an antigen or antibody on a multiwell plate (e.g. Polystyrene). The analyte is bound in the subsequent step.
The antigens are bound by the antibodies via hydrogen bonds, ionic and hydrophobic interactions, and Van-der-Waals forces. This binding occurs on a key-lock principle and is very specific. The antibodies used can thereby be monoclonal (mAb) or polyclonal (pAb). Monoclonal antibodies are made from hybridoma cells, a fusion of a cancer cell (myeloma) and an antibody-producing cell (B-cell) which selectively bind a specific epitope on the antigen. Polyclonal antibodies are a mixture of different antibodies which recognize different epitopes on the surface of one antigen. Once successfully attached the detection is normally made by colorimetry.
The successful antigen-antibody binding and addition of a substrate leads to a color change in the microtiter plate, which is catalyzed by an enzyme that has been previously conjugated to the antibody or antigen.
For further information, please refer to our Data Library at any time.