×
Account confirmation is required. Please, check your email for the confirmation link.
First name is required!
Last name is required!
First name is not valid!
Last name is not valid!
This is not an email address!
Email address is required!
This email is already registered!
Password is required!
Enter a valid password!
Please enter 6 or more characters!
Please enter 16 or less characters!
Passwords are not same!
Terms and Conditions are required!
Email or Password is wrong!
Elisa
-
Sensitivity: 0.156 ng/ml
Detection: 0.049 ng/ml-200 ng/ml
Learn More
-
Sensitivity: 0.3 ng/ml
Detection: 0.9 ng/ml-14 ng/ml
Learn More
-
Sensitivity: 3.9 pg/ml
Detection: 15.6 pg/ml-1000 pg/ml
Learn More
-
Sensitivity: 15.161 µg/l
Detection: 40 µg/l-200 µg/l
Learn More
-
Sensitivity: 0.11 ng/ml
Detection: 0.45 ng/ml-30 ng/ml
Learn More
-
Sensitivity: 0.049 pg/ml
Detection: 0.049 pg/ml-200 pg/ml
Learn More
-
Sensitivity: 0.08 ng/ml
Detection: 0.312 ng/ml-20 ng/ml
Learn More
-
Sensitivity: 0.039 ng/ml
Detection: 0.156 ng/ml-10 ng/ml
Learn More
-
Sensitivity: 1.95 ng/ml
Detection: 7.8 ng/ml-500 ng/ml
Learn More
-
Sensitivity: 23.44 ng/ml
Detection: 93.75 ng/ml-6000 ng/ml
Learn More
-
Sensitivity: 1.56 ng/ml
Detection: 6.25 ng/ml-400 ng/ml
Learn More
-
Sensitivity: 15.63 ng/ml.
Detection: 31.25 ng/ml-2000 ng/ml
Learn More
-
Sensitivity: 19.5 pg/ml
Detection: 78 pg/ml-5000 pg/ml
Learn More
-
Sensitivity: 0.039 ng/ml
Detection: 0.156 ng/ml-10 ng/ml
Learn More
-
Sensitivity: 0.195 µg/ml
Detection: 0.78 ?g/ml-50 ?g/ml
Learn More
THE ELISA
Since 1971 ELISA is used for detection and or determining the concentration of proteins within analyte mixture, low molecular weight compounds (e.g. Toxins, hormones) or viruses.
The ELISA is based on an antibody-antigen detection. Basis of this is the immobilization of an antigen or antibody on a multiwell plate (e.g. Polystyrene). The analyte is bound in the subsequent step.
The antigens are bound by the antibodies via hydrogen bonds, ionic and hydrophobic interactions, and Van-der-Waals forces. This binding occurs on a key-lock principle and is very specific. The antibodies used can thereby be monoclonal (mAb) or polyclonal (pAb). Monoclonal antibodies are made from hybridoma cells, a fusion of a cancer cell (myeloma) and an antibody-producing cell (B-cell) which selectively bind a specific epitope on the antigen. Polyclonal antibodies are a mixture of different antibodies which recognize different epitopes on the surface of one antigen. Once successfully attached the detection is normally made by colorimetry.
The successful antigen-antibody binding and addition of a substrate leads to a color change in the microtiter plate, which is catalyzed by an enzyme that has been previously conjugated to the antibody or antigen.
For further information, please refer to our Data Library at any time.