Human IL3RA / CD123 ELISA Pair Set

Brand

Sino Biological

short description

The human IL3RA / CD123 ELISA Pair Set is for the quantitative determinationof human IL3RA / CD123. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for IL3RA / CD123 coated on a 96-well plate. Standards and samples are added to the wells,and any IL3RA / CD123 present binds to the immobilized antibody. The wells arewashed and a horseradish peroxidase conjugated rabbit anti-IL3RA / CD123 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of IL3RA / CD123 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of themicrowell are read at 450 nm.

spezie

Human

detection target

IL3Ra
Standard (antigene): Each vial contains 65 ng of recombinant IL3RA. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 2 ng/mL is recommended.
capture antibody type: 0.35 mg/mL of mouse anti-IL3RA monoclonal antibody. Dilute to a working concentration of 2 µg/mL in CBS before coating. (Catalog: # 10518-MM01)
detection antibody type: 0.5 mg/mL rabbit anti-IL3RA monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 µg/mL in detection antibody dilution buffer before use. (Catalog: # 10518-R012)
Lable: HRP

sensitivity

The minimum detectable dose of Human IL3RA / CD123 was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

Detection limitation

31.25 pg/ml

certificate

ISO9001-2008

pack size

5 and 15 Plates

storage and Stability

Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20°C to -80°C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.