Bovine Alpha-Lactalbumin (aLA) ELISA Kit

Brand

Reddot Biotech

short description

for quantitatively determining the concentration in the customer's samples
Intended Use: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of this index in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

host

Bos taurus; Bovine Cattle

spezie

Bovine

Cross Reaction

This assay has high sensitivity and excellent specificity for detection of this index.
No significant cross-reactivity or interference between this index and analogues was observed.

detection target

aLA

sensitivity

0.32ng/mL

detection range

0.781-50ng/mL

pack size

48 and 96 Wells

storage and Stability

1.For unopened kit: All the reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer (30 � concentrate) and the Stop Solution should be stored at 4oC upon receipt while the others should be at -20oC. 2.For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Principle of the Elisa

The microtiter plate provided in this kit has been pre-coated with an antibody specific to this index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to this index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain this index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ﾱ 10nm. The concentration of this index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Kit Content

Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 2
Standard 2 Standard Diluent 1x20mL
Detection Reagent A 1x120µL Assay Diluent A 1x12mL
Detection Reagent B 1x120µL Assay Diluent B 1x12mL
TMB Substrate 1x9mL Stop Solution 1x6mL
Wash Buffer (30 x concentrate) 1x20mL Instruction manual 1