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Boster
For quantitative detection of rat IL-13 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Rat
There is no detectable cross-reactivity with other relevant proteins.
Rat IL-13Protein name: Interleukin-13Gene Full Name: interleukin 13abbreviation: IL13Background: Interleukin 13(IL-13) is a protein that in humans is encoded by the IL13 gene. IL-13 is cytokine secreted by many cell types, but especially T helper type 2(Th2) cells, that is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor(IL-4Ralpha) and at least one of two known IL-13-specific binding chains. Most of the biological effects of IL-13, like those of IL-4, are linked to a single transcription factor, signal transducer and activator of transcription 6(STAT6). The IL13 gene is clustered with IL3, IL5, IL4, and CSF2 on 5q. Physical maps of the 5q23-q31 region show the following order: cen--IL3--CSF2--IL13--IL4--IL5--tel, with IL13 particularly close to IL4. ID:P42203 ID:116553
Allergic rhinitis|ALRH|BHR 1|BHR1|Bronchial hyperresponsiveness 1 (bronchial asthma)|IL 13|IL-13|Il13|IL13_HUMAN|Interleukin 13|Interleukin-13|Interleukin13|MGC116786|MGC116788|MGC116789|NC30|P600
Nativ and recombinant rat IL-13
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
E.coli, T19-H131
<1pg/ml
15.6pg/ml-1000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|174|396|648|284|520|706 Standard deviation|7.66|24.95|35|14.77|39|46.6 CV(%)|4.4|6.3|5.4|5.2|7.5|6.6
25-30
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s rat IL-13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-13 has been precoated onto 96-well plates. Standards(E.coli, T19-H131) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-13 amount of sample captured in plate.
Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml rat IL-13 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each rat IL-13 standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000 O.D.|0.156|0.186|0.237|0.326|0.512|0.866|1.434|2.107
Catalog number|Description|Quantity-|96-well plate precoated with anti- rat IL-13 antibody|1ST0000-10|Lyophilized recombinant rat IL-13 standard|10ng/tube�2AR1107|Biotinylated anti- rat IL-13 antibody|130µl(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)AR1106-1|Sample diluent buffer|30 mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Pack size | 1x96 Wells |
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Brand | Boster |
Country of Manufacture | USA |
Sensitivity | <1pg/ml |
Specific Against | Rat |
Antigen Name | Interleukin-13 (IL13) |
Detection Range | 15.6pg/ml-1000pg/ml |
Alias | ALRH, BHR 1, BHR1, Bronchial hyperresponsiveness 1 (bronchial asthma), IL 13, IL-13, Il13, IL13_HUMAN, Interleukin 13, Interleukin-13, Interleukin13, MGC116786, MGC116788, MGC116789, NC30, P600, T-cell activation protein P600 |