Rat IL-1 beta PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of rat IL-1 beta in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Rat

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Rat IL-1 beta
Protein name: Interleukin-1 beta
Gene Full Name: interleukin 1, beta
abbreviation: IL1B
Background: The Interleukin 1 family is a group of 11 beta trefoil cytokines. Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response . Interleukin-1beta(IL-1beta) is a potent stimulator of bone resorption, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta belongs to proinflammatory cytokines, promote cancer cell adhesion and liver metastases by up-regulating the expression of vascular cell adhesion molecule-1(VCAM-1) on hepatic sinusoidal endothelium(HSE).
ID:Q63264
ID:24494

alias

Catabolin| Hematopoietin 1| IFN beta inducing factor| IL 1| IL 1 beta| IL 1B| IL-1 beta| IL1| IL1 BETA| IL1B| IL1B_HUMAN| IL1F2| Interleukin 1 beta| Interleukin 1 beta precursor| Interleukin-1 beta| LAF| OAF| Osteoclast activating factor| Preinterleukin beta| Pro interleukin 1 beta

Origin

Nativ and recombinant rat IL-1 beta

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

E.coli,V117-S268

sensitivity

<1pg/ml

detection range

31.2pg/ml-2000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|105|756|1359|112|688|1452
Standard deviation|3.99|34.78|66.59|5.712|37.84|92.93
CV(%)|3.8|4.6|4.9|5.1|5.5|6.4

TMB(tetramethylbenzidine) incubation time

25-30

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s rat IL-1 beta ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-1 beta has been precoated onto 96-well plates. Standards (E.coli,V117-S268) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1 beta is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-1 beta amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml rat IL-1 beta standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. We recommend that each rat IL-1 betastandard solution and each sample is measured in duplicate.

tipical result

Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000 O.D.|0.022|0.072|0.136|0.213|0.364|0.779|1.626|2.440

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- rat IL-1 beta antibody|1
ST0000-10|Lyophilized recombinant rat IL-1 beta standard|10ng/tubex2
AR1107|Biotinylated anti- rat IL-1 beta antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4