Rat CD86/B7-2 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of rat CD86 in cell culture supernates and serum.

spezie

Rat

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Rat CD86
Protein name: T-lymphocyte activation antigen CD86
Gene Full Name: CD86 molecule
abbreviation: CD86
Background: Cluster of Differentiation 86(also known as CD86 and B7-2) is aprotein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21 and mouse chromosome 16, band B5. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.
ID:Q62810
ID:56822

alias

Activation B7 2 antigen|B7 2|B70|B72|BU63|CD28LG2|CD86 antigen (CD28 antigen ligand 2 B7 2 antigen)|CD86 molecule|CTLA 4 counter receptor B7.2|FUN 1|FUN1|T lymphocyte activation antigen CD86 precursor

Origin

Nativ and recombinant rat CD86

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, V29-I250

sensitivity

<10pg/ml

detection range

62.5pg/ml-4000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|0.21|1.44|2.59|0.23|1.47|2.46
Standard deviation|0.010|0.102|0.161|0.013|0.118|0.182
CV(%) |4.8|7.1|6.2|5.8|8.0|7.4

TMB(tetramethylbenzidine) incubation time

25-30

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s rat CD86 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD86 has been precoated onto 96-well plates. Standards(NSO, V29-I250) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD86 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CD86 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml rat CD86 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates or serum to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each rat CD86 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000 O.D.|0.058|0.135|0.208|0.365|0.614|0.986|1.727|2.367

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- rat CD86 antibody|1
ST0000-10|Lyophilized recombinant rat CD86 standard|10ng/tube�2
AR1107|Biotinylated anti- rat CD86 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4