Rat CCL11/Eotaxin PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of rat Eotaxin in cell culture supernates, serum and plasma(EDTA, citrate).

spezie

Rat

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

rat Eotaxin
Protein name: Eotaxin
Gene Full Name: chemokine (C-C motif) ligand 11
abbreviation: CCL11
Background: Eotaxin, also known as CCL11, is a potent inducer of eosinophil chemotaxis and is considered as a selective ligand of the CC chemokine receptor 3 (CCR3), which is expressed on eosinophils, basophils, and Th2 lymphocytes. Eotaxin is assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. The gene maps to chromosome 17 and is expressed constitutively at high levels in small intestine and colon, and at lower levels in various other tissues. The deduced mature protein sequence is 66% identical to human monocyte chemoattractant protein-1, and 60% identical to guinea pig eotaxin.
ID:P97545
ID:29397

alias

C-C motif chemokine 11|CCL 11|CCL11|CCL11_HUMAN|Chemokine (C-C motif) ligand 11|Chemokine C C motif ligand 11|Chemokine CC Motif Ligand 11|Chemokine ligand 11|Eosinophil Chemotactic P|Eosinophil chemotactic protein|Eotaxin|Eotaxin 1|SCYA 11|SCYA11

Origin

Nativ and recombinant rat Eotaxin

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

E.coli, H24-P97

sensitivity

<10pg/ml

detection range

15.6pg/ml-1000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|74.6|267|646|101.4|309|718
Standard deviation|3.80|14.55|36.18|6.49|22.25|56.72
CV(%)|5.1|6.2|5.6|6.4|7.2|7.9

TMB(tetramethylbenzidine) incubation time

15-20

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s rat Eotaxin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Eotaxin has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: H24-P97) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Eotaxin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Eotaxin amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml rat Eotaxin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernatants, serum or plasma(EDTA, citrate) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each rat Eotaxin standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000 O.D.|0.015|0.104|0.151|0.286|0.579|1.050|2.051|2.562

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- rat Eotaxin antibody|1
ST0000-10|Lyophilized recombinant rat Eotaxin standard|10ng/tube�2
AR1107|Biotinylated anti- rat Eotaxin antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4