Profile Informations
Login Datas
or login
Boster
For quantitative detection of rat BTC in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Rat
There is no detectable cross-reactivity with other relevant proteins.
rat BTCProtein name: ProbetacellulinGene Full Name: betacellulinabbreviation: BTCBackground: Betacellulin, also known as BTC is a protein that in humans is encoded by the BTC gene. This gene is mapped to 4q13.3. The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. It is the growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. This protein is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. ID:Q9JJM4 ID:64022
Betacellulin precursor|BTC|Probetacellulin
Nativ and recombinant rat BTC
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
E.coli, D32-Y111
<10pg/ml
7.8pg/ml-500pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|95|178|362|105|241|413 Standard deviation|3.61|7.3|16.29|4.62|12.53|23.13 CV(%)|3.8|4.1|4.5|4.4|5.2|5.6
25-30
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s rat BTC ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BTC has been precoated onto 96-well plates. Standards(E.coli, D32-Y111) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BTC is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat BTC amount of sample captured in plate.
Aliquot 0.1ml per well of the 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml, 7.8pg/ml rat BTC standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, serum, plasma(heparin, EDTA), tissue homogenates or urine to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each rat BTC standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|7.8|15.6|31.3|62.5|125|250|500 O.D.|0.100|0.118|0.128|0.171|0.253|0.421|0.837|1.801
Catalog number|Description|Quantity-|96-well plate precoated with anti- rat BTC antibody|1ST0000-10|Lyophilized recombinant rat BTC standard|10ng/tube�2AR1107|Biotinylated anti- rat BTC antibody|130µl(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)AR1106-1|Sample diluent buffer|30mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Pack size | 1x96 Wells |
---|---|
Brand | Boster |
Country of Manufacture | USA |
Sensitivity | <10pg/ml |
Specific Against | Rat |
Antigen Name | Probetacellulin (BTC) |
Detection Range | 7.8pg/ml-500pg/ml |
Alias | Betacellulin precursor, BTC, Probetacellulin |