Rat BMP-7 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of rat BMP-7 in bone tissue, cell culture supernates, serum and plasma((heparin, EDTA).

spezie

Rat

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

rat BMP-7
Protein name: Bone morphogenetic protein 7
Gene Full Name: bone morphogenetic protein 7
abbreviation: BMP7
Background: Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-β superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.
ID:Q9R228
ID:85272

alias

BMP 7|BMP7|BMP-7|Bone morphogenetic protein 7|Eptotermin alfa|OP 1|OP1|OP1-|Osteogenic protein 1

Origin

Nativ and recombinant rat BMP-7

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

CHO, S292-H430

sensitivity

<10pg/ml

detection range

31.2pg/ml-2000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|135|609|1288|198|716|1563
Standard deviation|6.08|32.28|66.98|15.05|58.71|109.41
CV(%)|4.5|5.3|5.2|7.6|8.2|7.0

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s rat BMP-7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BMP-7 has been precoated onto 96-well plates. Standards(Expression system for standard: CHO, Immunogen sequence: S292-H430) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat BMP-7 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml rat BMP-7 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat bone tissue, cell culture supernates, serum or plasma((heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each rat BMP-7 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000 O.D.|0.084|0.129|0.171|0.233|0.391|0.712|1.320|2.240

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- rat BMP-7 antibody|1
ST0000-10|Lyophilized recombinant rat BMP-7 standard|10ng/tube�2
AR1107|Biotinylated anti- rat BMP-7 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4