Profile Informations
Login Datas
or login
Boster
For quantitative detection of rat BMP-2 in bone tissue, cell culture supernates and serum.
Rat
There is no detectable cross-reactivity with other relevant proteins.
Rat BMP-2Protein name: Bone morphogenetic protein 2Gene Full Name: bone morphogenetic protein 2abbreviation: BMP2Background: Bone morphogenetic protein-2(BMP-2, BMP 2A) belongs to the transforming growth factor beta(TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice. BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase(MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis. Bone morphogenic proteins(BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion. The standard product used in this kit is recombinant rat BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa. ID:P49001 ID:29373
BDA2|BMP-2|BMP-2A|Bmp2|BMP2_HUMAN|BMP2A|Bone morphogenetic protein 2|Bone morphogenetic protein 2A
Nativ and recombinant rat BMP-2
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
CHO, Q283-R396
<2pg/ml
31.2pg/ml-2000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|285|742|1197|306|834|1358 Standard deviation|12.25|37.84|51.47|16.21|45.87|55.67 CV(%)|4.3|5.1|4.3|5.3|5.5|4.1
15-20
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s rat BMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BMP-2 has been precoated onto 96-well plates. Standards(CHO, Q283-R396) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat BMP-2 amount of sample captured in plate.
Aliquot 0.1ml per well of the 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml rat BMP-2 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat bone tissue, cell culture supernates or serum to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each rat BMP-2 standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000 O.D.|0.017|0.069|0.116|0.215|0.454|0.992|2.008|2.506
Catalog number|Description|Quantity-|96-well plate precoated with anti- rat BMP-2 antibody|1ST0000-10|Lyophilized recombinant rat BMP-2 standard|10ng/tube�2AR1107|Biotinylated anti- rat BMP-2 antibody|130µl(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)AR1106-1|Sample diluent buffer|30 mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Pack size | 1x96 Wells |
---|---|
Brand | Boster |
Country of Manufacture | USA |
Sensitivity | <2pg/ml |
Specific Against | Rat |
Antigen Name | Bone morphogenetic protein 2 (BMP2) |
Detection Range | 31.2pg/ml-2000pg/ml |
Alias | BDA2, BMP-2, BMP-2A, Bmp2, BMP2_HUMAN, BMP2A, Bone morphogenetic protein 2, Bone morphogenetic protein 2A |