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Boster
For quantitative detection of rat Adiponectin in cell culture supernates, serum, plasma(heparin, EDTA), tissue homogenates and urine.
Rat
There is no detectable cross-reactivity with other relevant proteins.
rat AdiponectinProtein name: AdiponectinGene Full Name: adiponectin, C1Q and collagen domain containingabbreviation: ADIPOQBackground: Adiponectin(ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin. ID:Q8K3R4 ID:246253
30 kDa adipocyte complement related protein| 30 kDa adipocyte complement-related protein| ACDC| ACRP30| ADIPO_HUMAN| Adipocyte| Adipocyte C1q and collagen domain containing protein| Adipocyte complement related 30 kDa protein| Adipocyte complement related protein of 30 kDa| Adipocyte complement-related 30 kDa protein| Adiponectin| Adipoq| Adipose most abundant gene transcript 1| Adipose most abundant gene transcript 1 protein| Adipose specific collagen like factor| ADIPQTL1| ADPN| apM-1| APM1| C1q and collagen domain-containing protein| GBP28| Gelatin binding protein| Gelatin binding protein 28| Gelatin-binding protein
Nativ and recombinant rat Adiponectin
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
E.coli, I19-N244
<10pg/ml
156pg/ml-10000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(ng/ml)|0.6|3.5|6.7|1.2|3.9|5.7 Standard deviation|0.027|0.112|0.261|0.114|0.277|0.49 CV(%)|4.5|3.2|3.9|9.5|7.1|8.6
25-30
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s rat Adiponectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Adiponectin has been precoated onto 96-well plates. Standards(E.coli, I19-N244) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Adiponectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Adiponectin amount of sample captured in plate.
Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml rat Adiponectin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, serum, plasma(heparin, EDTA), tissue homogenates or urine to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each rat Adiponectin standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000 O.D.|0.004|0.086|0.164|0.305|0.588|0.961|1.511|1.937
Catalog number|Description|Quantity-|96-well plate precoated with anti- rat Adiponectin antibody|1ST0000-20|Lyophilized recombinant rat Adiponectin standard|20ng/tube�2AR1107|Biotinylated anti- rat Adiponectin antibody|130µl(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)AR1106-1|Sample diluent buffer|30mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Pack size | 1x96 Wells |
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Brand | Boster |
Country of Manufacture | USA |
Sensitivity | <10pg/ml |
Specific Against | Rat |
Antigen Name | Adiponectin (ADIPOQ) |
Detection Range | 156pg/ml-10000pg/ml |
Alias | 30 kDa adipocyte complement related protein, 30 kDa adipocyte complement-related protein, ACDC, ACRP30, ADIPO_HUMAN, Adipocyte, Adipocyte C1q and collagen domain containing protein, Adipocyte complement related 30 kDa protein, Adipocyte compleme |