Rat Activin A PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of rat Activin A in cell culture supernates, serum, plasma(heparin, EDTA) and saliva.

spezie

Rat

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Rat Activin A
Protein name: Inhibin beta A chain
Gene Full Name: inhibin, beta A
abbreviation: INHBA
Background: Activin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules. Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types. Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa.
ID:P18331
ID:29200

alias

Activin beta A chain|erythroid differentiation factor|erythroid differentiation protein|follicle stimulating hormone releasing protein|FSH releasing protein|Inhibin|inhibin beta A chain

Origin

Nativ and recombinant rat Activin A

Antibodies

a monoclonal antibody from mouse|a biotinylated monoclonal antibody from mouse

Immunogene

CHO, G311-S426

sensitivity

<12pg/ml

detection range

15.6pg/ml-1000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|97|294|459|103|315|512
Standard deviation|4.9|14.7|23.9|8.0|19.8|24.6
CV(%)|5|5|5.2|7.8|6.3|4.8

TMB(tetramethylbenzidine) incubation time

25-30

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s rat Activin A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Activin A has been precoated onto 96-well plates. Standards(CHO, G311-S426) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for Activin A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Activin A amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml rat Activin A standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, serum, plasma(heparin, EDTA) or saliva to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each rat Activin A standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000 O.D.|0.028|0.049|0.111|0.175|0.328|0.851|1.457|2.491

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- rat Activin A antibody|1
ST0000-10|Lyophilized recombinant rat Activin A standard|10ng/tubex2
AR1107|Biotinylated anti- rat Activin A antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4