QuickTiter™ MuLV Core Antigen ELISA Kit

Brand

Cell Biolabs

short description

An anti-MuLV p30 monoclonal coating antibody is adsorbed onto a microtiter plate. MuLV core antigen (p30) present in the sample or standard binds to the antibodies adsorbed on the plate; an anti-MuLV p30 polyclonal antibody is added and binds to the antigen captured by the first antibody. Following incubation and wash steps, a HRP-conjugated secondary antibody is added and binds to the anti-MuLV p30 polyclonal. Unbound HRP-conjugated secondary antibody is removed during the wash steps, and substrate solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of MuLV core antigen present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from recombinant MuLV p30 core antigen and sample concentration is then determined.
Research area: Pathogen and Toxin Assays
Research focus Virus Core Antigen Detection

spezie

other

detection target

MuLV Core Antigen

Origin

Recombinant Protein purified from E. coli

sensitivity

Measures the MuLV core protein (p30) as low as 300 pg/mL

pack size

96 assays

storage and Stability

Upon receipt, aliquot and store the Recombinant MuLV p30 Standard at -20°C to avoid multiple freeze/thaw cycles. Store all other kit components at 4°C.

Shipping Temp

Blue Ice

Kit Content

Recombinant MuLV p30 Standard included