Phospho-ERK/JNK/P38? ELISA

Brand

Raybiotech

short description

RayBio® Phospho-ERK/JNK/P38? ELISA Kit. This assay semi-quantitatively measures phophorylated ERK/JNK/P38? in lysate samples.

Cross Reaction

Human, Mouse, Rat

detection target

ERK1 (P-T202/Y204, JNK (P-Thr183/Tyr185), P38 (P-Thr180/Tyr182)

alias

Mitogen-activated protein kinase 3 (MAP kinase 3) (MAPK 3) (EC 2.7.11.24) (ERT2) (Extracellular signal-regulated kinase 1) (ERK-1) (Insulin-stimulated MAP2 kinase) (MAP kinase isoform p44) (p44-MAPK) (Microtubule-associated protein 2 kinase) (p44-ERK1) Mitogen-activated protein kinase 1 (MAP kinase 1) (MAPK 1) (EC 2.7.11.24) (ERT1) (Extracellular signal-regulated kinase 2) (ERK-2) (MAP kinase isoform p42) (p42-MAPK) (Mitogen-activated protein kinase 2) (MAP kinase 2) (MAPK 2)

certificate

GMP/ISO

pack size

1x96, 2x96 and 5x96 Wells Microplate Kit

storage and Stability

Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Principle of the Elisa

Phosphorylation ELISA kits - Phosphorylation ELISA

Kit Content

Pre-Coated 96-well Strip Microplate; Wash Buffer; Anti-Phospho Antibody; HRP-Conjugated Secondary Antibody; Assay Diluent; TMB One-Step Substrate; Stop Solution; Lysis Buffer; Positive Control Sample