Mouse M-CSF PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of mouse M-CSF in cell culture supernates, tissue homogenates, serum and plasma (EDTA).

spezie

Mouse

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Mouse M-CSF
Protein name: Macrophage colony-stimulating factor 1
Gene Full Name: colony stimulating factor 1
abbreviation: CSF1
Background: M-CSF, also called CSF1, consists of a 14-amino acid peptide, longer than the usual 8-to-11 mer recognized by most CTLs. The M-CSF gene is mapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb1. Although it is a single-copy gene, its expression results in the synthesis of several mRNAs, ranging in size from about 1.5 to 4.5 kb2. There are 2 forms of M-CSF, with 224 and 522 amino acids, resulting from alternative splicing3. Furthermore, M-CSF has a role in development of the placenta. Uterine M-CSF concentration is regulated by a synergistic action of estradiol and progesterone. M-CSF is produced by uterine glandular epithelial cells. It had been found that FMS, the M-CSF receptor, is expressed in placenta and choriocarcinoma cell lines4.
ID:P07141
ID:12977

alias

Colony stimulating factor 1 | CSF-1 | CSF1 | Lanimostim | MCSF | P09603 | CSF 1 | M CSF | M-CSF

Origin

Nativ and recombinant mouse M-CSF

Antibodies

a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat

Immunogene

E.coli, K33-E262

sensitivity

<1pg/ml

detection range

15.6pg/ml-1000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|92|316|628|127|439|685
Standard deviation|5.06|19.28|40.2|7.493|27.66|51.4
CV(%)|5.5|6.1|6.4|5.9|6.3|7.5

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s mouse M-CSF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for M-CSF has been precoated onto 96-well plates. Standards(E.coli, K33-E262) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for M-CSF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse M-CSF amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml mouse M-CSF standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, tissue lysates, serum or plasma (EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each mouse M-CSF standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000 O.D.|0.068|0.143|0.216|0.382|0.618|1.101|1.836|2.509

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- mouse M-CSF antibody|1
ST0000-10|Lyophilized recombinant mouse M-CSF standard|10ng/tubex2
AR1107|Biotinylated anti- mouse M-CSF antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4