Mouse IL1RL1/ST2 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of mouse IL1RL1 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Mouse

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

mouse IL1RL1
Protein name: Interleukin-1 receptor-like 1
Gene Full Name: interleukin 1 receptor-like 1
abbreviation: IL1RL1
Background: Interleukin 1 receptor-like 1, also known as IL1RL1 or ST2, is a protein that in humans is encoded by the IL1RL1 gene. The protein encoded by this gene is a member of the interleukin 1 receptor family. Studies of the similar gene in mouse suggested that this receptor can be induced by proinflammatory stimuli, and may be involved in the function of helper T cells. TL1RL1 is necessary for endotoxin tolerance and, by inhibiting TLR responses, enhances Th2 responses. This gene, interleukin 1 receptor, type I(IL1R1), interleukin 1 receptor, type II(IL1R2) and interleukin 1 receptor-like 2(IL1RL2) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.
ID:P14719
ID:17082

alias

DER-4|DER4|FIT 1|Growth stimulation expressed|homolog of mouse growth stimulation-expressed|Il1rl1|IL33R|ILRL1_HUMAN|Interleukin 1 receptor like 1|interleukin 1 receptor related protein|Interleukin-1 receptor-like 1|Ly84|Lymphocyte antigen 84|Protein ST2|ST2|ST2L|ST2V|Ste2|Suppressor of tumorigenicity 2|T1|T1 protein

Origin

Nativ and recombinant mouse IL1RL1

Antibodies

a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat

Immunogene

NSO, S27-R332

sensitivity

<10pg/ml

detection range

156pg/ml-10000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|0.57|2.33|4.25|0.68|2.74|4.61
Standard deviation|0.029|0.086|0.136|0.058|0.17|0.244
CV(%)|5.1|3.7|3.2|8.6|6.2|5.3

TMB(tetramethylbenzidine) incubation time

25-30

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s mouse IL1RL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL1RL1 has been precoated onto 96-well plates. Standards(NSO, S27-R332) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL1RL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL1RL1 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml mouse IL1RL1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each mouse IL1RL1 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000 O.D.|0.066|0.098|0.164|0.294|0.544|1.007|1.821|2.388

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- mouse IL1RL1 antibody|1
ST0000-10|Lyophilized recombinant mouse IL1RL1 standard|10ng/tube�2
AR1107|Biotinylated anti- mouse IL1RL1 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4