Mouse IL15RA PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of mouse IL15RA in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Mouse

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

mouse IL15RA
Protein name: Interleukin-15 receptor subunit alpha
Gene Full Name: interleukin 15 receptor, alpha
abbreviation: IL15RA
Background: Interleukin 15 receptor, alpha subunit is a subunit of the interleukin 15 receptor which is encoded by the gene IL15RA in humans. The human IL15RA gene was mapped to chromosome 10p15-p14 by FISH and the mouse Il15ra gene was mapped to chromosome 2. The cytoplasmic domain of IL15R-alpha, like that of IL2R-alpha, was dispensable for mitogenic signaling, suggesting that the primary role of the alpha chains is to confer high-affinity binding. At high concentrations, IL15, like IL2, was able to signal through a complex of IL2R-beta and -gamma in the absence of the alpha subunit. The soluble receptor displayed high affinity for IL15 and inhibited both binding of IL15 to the membrane receptor and IL15-induced cell proliferation.
ID:Q60819
ID:16169

alias

AA690181|CD215|I15RA_HUMAN|IL 15R alpha|IL-15 receptor subunit alpha|IL-15R-alpha|IL-15RA|IL15RA|Interleukin 15 receptor alpha|Interleukin 15 receptor subunit alpha|MGC104179|sIL-15 receptor subunit alpha|sIL-15R-alpha|sIL-15RA|Soluble interleukin 15 receptor subunit alpha|Soluble interleukin-15 receptor subunit alpha

Origin

Nativ and recombinant mouse IL15RA

Antibodies

a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat

Immunogene

NSO, G33-K205

sensitivity

<10pg/ml

detection range

125pg/ml-8000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|925|3080|5122|1273|3377|5419
Standard deviation|43.5|200.2|276.6|65|236.4|341.4
CV(%)|4.7|6.5|5.4|5.1|7|6.3

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s mouse IL15RA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Monoclonal antibody from rat specific for IL15RA has been precoated onto 96-well plates. Standards(NSO, G33-K205) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL15RA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL15RA amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 8000pg/ml, 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml mouse IL15RA standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each mouse IL15RA standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|125|250|500|1000|2000|4000|8000 O.D.|0.038|0.066|0.100|0.160|0.299|0.568|1.193|2.253

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- mouse IL15RA antibody|1
ST0000-10|Lyophilized recombinant mouse IL15RA standard|10ng/tube�2
AR1107|Biotinylated anti- mouse IL15RA antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4