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Boster
For quantitative detection of mouse IL-4 in cell culture supernates and serum.
Mouse
There is no detectable cross-reactivity with other relevant proteins.
Mouse IL-4Protein name: Interleukin-4Gene Full Name: interleukin 4abbreviation: IL4Background: Interleukin-4(IL-4), also knowns as a B-cell stimulatory factor1(BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells. The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis.1 The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. ID:P07750 ID:16189
B cell growth factor 1| B cell stimulatory factor 1| BCGF 1| BCGF1| Binetrakin| BSF 1| BSF1| IL 4| Interleukin 4| Lymphocyte stimulatory factor 1| MGC79402| Pitrakinra
Nativ and recombinant mouse IL-4
a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
E.coli, H21-S140
<1pg/ml
7.8pg/ml-500pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|44|168|283|57|185|326 Standard deviation|2.38|7.9|17.55|3.88|10.36|23.8 CV(%)|5.4|4.7|6.2|6.8|5.6|7.3
25-30
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s mouse IL-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-4 has been precoated onto 96-well plates. Standards(E.coli, H21-S140) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-4 amount of sample captured in plate.
Aliquot 0.1ml per well of the 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml, 7.8pg/ml mouse IL-4 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See モSample Dilution Guidelineヤ above for details. We recommend that each mouse IL-4 standard solution and each sample is measured in duplicate.
Concentration(pg/ml)|0|7.8|15.6|31.3|62.5|125|250|500 O.D.|0.003|0.039|0.072|0.138|0.310|0.786|1.681|2.362
Catalog number|Description|Quantity-|96-well plate precoated with anti- mouse IL-4 antibody|1ST0000-10|Lyophilized recombinant mouse IL-4 standard|10ng/tubex2AR1107|Biotinylated anti- mouse IL-4 antibody|130ul(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)AR1106-1|Sample diluent buffer|30mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Pack size | 1x96 Wells |
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Brand | Boster |
Country of Manufacture | USA |
Sensitivity | <1pg/ml |
Specific Against | Mouse |
Antigen Name | Interleukin-4 (IL4) |
Detection Range | 7.8pg/ml-500pg/ml |
Alias | B cell growth factor 1, B cell stimulatory factor 1, BCGF 1, BCGF1, Binetrakin, BSF 1, BSF1, IL 4, Interleukin 4, Lymphocyte stimulatory factor 1, MGC79402, Pitrakinra |