Mouse IL-33 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of mouse IL-33 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Mouse

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Mouse IL-33
Protein name: Interleukin-33
Gene Full Name: interleukin 33
abbreviation: IL33
Background: Interleukin 33(IL-33) is a cytokine belonging to the IL-1 superfamily. By genomic sequence analysis, the IL33 gene is mapped to chromosome 9p24.1 and its mouse homolog to a syntenic region on chromosome 19qC1. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33. IL33, an alarmin released from necrotic cells, is necessary for potent CD8 + T cell(CTL) responses to replicating, prototypic RNA, and DNA viruses in mice. IL33 prevented the downregulation of CXCR2 and inhibition of chemotaxis induced by activation of TLR4, and found that IL33 reverses the TLR4-induced reduction of CXCR2 expression via the inhibition of expression of GRK2.
ID:Q8BVZ5
ID:77125

alias

C9orf26| DVS27| DVS27 related protein| IL-1F11| IL-33| IL1F11| IL33| IL33_HUMAN| Interleukin-1 family member 11| Interleukin-33| NF-HEV| NFEHEV| Nuclear factor from high endothelial venules| RP11-575C20.2

Origin

Nativ and recombinant mouse IL-33

Antibodies

a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat

Immunogene

E.coli, S109-I266

sensitivity

<10pg/ml

detection range

31.2pg/ml-2000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|118|547|1240|245|644|1435
Standard deviation|8.38|32.82|68.2|18.13|39.93|83.23
CV(%)|7.1|6|5.5|7.4|6.2|5.8

TMB(tetramethylbenzidine) incubation time

15-20

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s mouse IL-33 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-33 has been precoated onto 96-well plates. Standards(E.coli, S109-I266) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-33 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-33 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml mouse IL-33 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each mouse IL-33 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000 O.D.|0.009|0.068|0.117|0.224|0.401|0.775|1.405|2.212

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- mouse IL-33 antibody|1
ST0000-10|Lyophilized recombinant mouse IL-33 standard|10ng/tubex2
AR1107|Biotinylated anti- mouse IL-33 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4