Mouse IL-23 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of mouse IL-23 in cell culture supernates, serum and plasma(heparin).

spezie

Mouse

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Mouse IL-23
Protein name: Interleukin-23 subunit alpha
Gene Full Name: interleukin 23, alpha subunit p19
abbreviation: IL23A
Background: Interleukin-23 subunit alpha(IL-23) is a protein that in humans is encoded by the IL23A gene. IL-23 is a heterodimeric cytokine consisting of two subunits, one called p40, which is shared with another cytokine, IL-12, and another called p19(the IL-23 alpha subunit). IL-23 is an important part of the inflammatory response against infection. It promotes upregulation of the matrix metalloprotease MMP9, increases angiogenesis and reduces CD8+ T-cell infiltration. Recently, IL-23 has been implicated in the development of cancerous tumors. he International Radiation Hybrid Mapping Consortium mapped the p19 gene to chromosome 12(stSG47812).
ID:Q9EQ14
ID:83430

alias

IL 23| IL 23A| IL-23 subunit alpha| IL-23-A| IL-23p19| Il23a| IL23A_HUMAN| IL23P19| Interleukin 23 alpha subunit p19| Interleukin-23 subunit alpha| Interleukin-23 subunit p19| JKA3 induced upon T cell activation| P19| SGRF

Origin

Nativ and recombinant mouse IL-23

Antibodies

a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat

Immunogene

sf21, (M23-S335)+(L20-A196)

sensitivity

<2pg/ml

detection range

31.2pg/ml-2000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|255|717|1147|325|836|1327
Standard deviation|11.73|40.87|68.82|18.85|53.5|95.5
CV(%)|4.6|5.7|6|5.8|6.4|7.2

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s mouse IL-23 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-23 has been precoated onto 96-well plates. Standards(sf21, (M23-S335)+(L20-A196)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-23 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-23 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml mouse IL-23 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatant, serum or plasma (heparin) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each mouse IL-23 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000 O.D.|0.168|0.198|0.242|0.355|0.547|0.935|1.561|2.391

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- mouse IL-23 antibody|1
ST0000-10|Lyophilized recombinant mouse IL-23 standard|10ng/tube�2
AR1107|Biotinylated anti- mouse IL-23 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4