Mouse IL-21 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of mouse IL-21 in cell culture supernates and serum.

spezie

Mouse

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Mouse IL-21
Protein name: Interleukin-21
Gene Full Name: interleukin 21
abbreviation: IL21
Background: Interleukin-21 is a protein that in humans is encoded by the IL21 gene. Interleukin 21 is a cytokine that has potent regulatory effects on cells of the immune system, including natural killer(NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells. By radiation hybrid analysis, the IL21 gene is mapped to chromosome 4q26-q27, near the IL2 gene. IL21 stimulates B-cell proliferation in an anti-CD40-dependent manner but inhibits proliferation stimulated by IL4 plus anti-IgM. The biologic effects of IL21 on NK cells differ between species, with human IL21 enhancing NK cell proliferation and murine IL21 inhibiting NK cell proliferation. IL21 is a cytokine highly expressed by mouse T(H)17 cells.
ID:Q9ES17
ID:60505

alias

Cytokine CX2| DN 452A22.7| IL 21| Interleukin 21| Interleukin 21 isoform| Interleukin21| OTTMUSP00000007838| Za11

Origin

Nativ and recombinant mouse IL-21

Antibodies

a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat

Immunogene

E.coli, P25-S146

sensitivity

<10pg/ml

detection range

62.5pg/ml-4000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|316|1296|2113|427|1450|2485
Standard deviation|15.8|80.4|99.31|25.2|97.15|159
CV(%)|5|6.2|4.7|5.9|6.7|6.4

TMB(tetramethylbenzidine) incubation time

15-20

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s mouse IL-21 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-21 has been precoated onto 96-well plates. Standards(E.coli, P25-S146) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-21 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-21 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml mouse IL-21 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each mouse IL-21 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000 O.D.|0.061|0.093|0.131|0.239|0.479|0.837|1.677|2.361

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- mouse IL-21antibody|1
ST0000-10|Lyophilized recombinant mouse IL-21 standard|10ng/tube�2
AR1107|Biotinylated anti- mouse IL-21 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4