Mouse IL-17D PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of mouse IL-17D in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Mouse

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Mouse IL-17D
Protein name: Interleukin-17D
Gene Full Name: interleukin 17D
abbreviation: IL17D
Background: Interleukin 17D (IL-17D) is a member of the unique IL-17 family, which share a conserved cysteine motif. It is mapped to 13q12.11. IL-17D regulates cytokine production in endothelial cells and inhibits hemopoiesis in vitro. Purified recombinant IL-17D inhibited myeloid progenitor cell colony formation. IL-17D can enhance endothelial cell production of IL6, IL8, and GMCSF, but not other cytokines. And it is preferentially expressed in skeletal muscle, brain, adipose tissue, heart, lung, and pancreas.
ID:Q8K4C4
ID:239114

alias

IL 17D|IL 22|IL 27|IL-17D|IL-27|IL17D|IL17D_HUMAN|IL27|Interleukin 17D|Interleukin 27|Interleukin-17D|Interleukin-27

Origin

Nativ and recombinant mouse IL-17D

Antibodies

a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat

Immunogene

E.coli, A25-R205

sensitivity

<10pg/ml

detection range

62.5pg/ml-4000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|715|1410|2703|694|1582|2691
Standard deviation|30.03|64.86|137.9|38.17|99.67|180.3
CV(%)|4.2|4.6|5.1|5.5|6.3|6.7

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s mouse IL-17D ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-17D has been precoated onto 96-well plates. Standards(E.coli, A25-R205) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17D is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-17D amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml mouse IL-17D standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each mouse IL-17D standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000 O.D.|0.042|0.106|0.158|0.253|0.460|0.883|1.530|2.288

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- mouse IL-17D antibody|1
ST0000-10|Lyophilized recombinant mouse IL-17D standard|10ng/tubex2
AR1107|Biotinylated anti- mouse IL-17D antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4