Profile Informations
Login Datas
or login
Boster
For quantitative detection of mouse IL-10 in cell culture supernates and serum.
Mouse
There is no detectable cross-reactivity with other relevant proteins.
Mouse IL-10Protein name: Interleukin-10Gene Full Name: interleukin 10abbreviation: IL10Background: Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation. The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease. The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1. The standard product used in this kit is recombinant mouse IL-10, consisting of 160 amino acid with the molecular mass of 18KDa. ID:P18893 ID:16153
CSIF|Cytokine synthesis inhibitory factor|GVHDS|IL 10|IL-10|IL10|IL10_HUMAN|IL10A|Interleukin 10|Interleukin-10|Interleukin10|MGC126450|MGC126451|T cell growth inhibitory factor|TGIF
Nativ and recombinant mouse IL-10
a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
E.coli, S19-S178
<1pg/ml
15.6pg/ml-1000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|82|413|724|113|367|628 Standard deviation|3.77|21.5|44.2|7|24.96|46.5 CV(%)|4.6|5.2|6.1|6.2|6.8|7.4
25-30
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s mouse IL-10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-10 has been precoated onto 96-well plates. Standards(E.coli, S19-S178) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-10 amount of sample captured in plate.
Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml mouse IL-10 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each mouse IL-10 standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000 O.D.|0.055|0.129|0.184|0.322|0.507|0.943|1.294|1.905
Catalog number|Description|Quantity-|96-well plate precoated with anti- mouse IL-10 antibody|1ST0000-10|Lyophilized recombinant mouse IL-10 standard|10ng/tubex2AR1107|Biotinylated anti- mouse IL-10 antibody|130ul(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)AR1106-1|Sample diluent buffer|30mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Pack size | 1x96 Wells |
---|---|
Brand | Boster |
Country of Manufacture | USA |
Sensitivity | <1pg/ml |
Specific Against | Mouse |
Antigen Name | Interleukin-10 (IL10) |
Detection Range | 15.6pg/ml-1000pg/ml |
Alias | CSIF, Cytokine synthesis inhibitory factor, GVHDS, IL 10, IL-10, IL10, IL10_HUMAN, IL10A, Interleukin 10, Interleukin-10, Interleukin10, MGC126450, MGC126451, T cell growth inhibitory factor, TGIF |