Mouse IL-1 beta PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of mouse IL-1 beta in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Mouse

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Mouse IL-1 beta
Protein name: Interleukin-1 beta
Gene Full Name: interleukin 1, beta
abbreviation: IL1B
Background: Interleukin-1beta(IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B(NFKB)-regulated genes2. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.
ID:P10749
ID:16176

alias

Catabolin| Hematopoietin 1| IFN beta inducing factor| IL 1| IL 1 beta| IL 1B| IL-1 beta| IL1| IL1 BETA| IL1B| IL1B_HUMAN| IL1F2| Interleukin 1 beta| Interleukin 1 beta precursor| Interleukin-1 beta| LAF| OAF| Osteoclast activating factor| Preinterleukin beta| Pro interleukin 1 beta

Origin

Nativ and recombinant mouse IL-1 beta

Antibodies

a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat

Immunogene

E.coli,V118-S269

sensitivity

<1pg/ml

detection range

12.5pg/ml-800pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|86|376|587|79|346|558
Standard deviation|6.97|19.55|24.07|7.03|25.6|37.94
CV(%)|8.1|5.2|4.1|8.9|7.4|6.8

TMB(tetramethylbenzidine) incubation time

25-30

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s mouse IL-1 beta ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-1 beta has been precoated onto 96-well plates. Standards (E.coli,V118-S269) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1 beta is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-1 beta amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 800pg/ml, 400pg/ml, 200pg/ml, 100pg/ml, 50pg/ml, 25pg/ml, 12.5pg/ml mouse IL-1 beta standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. We recommend that each mouse IL-1 betastandard solution and each sample is measured in duplicate.

tipical result

Concentration(pg/ml)|0|12.5|25|50|100|200|400|800 O.D.|0.063|0.107|0.143|0.214|0.390|0.702|1.285|2.092

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- mouse IL-1 beta antibody|1
ST0000-10|Lyophilized recombinant mouse IL-1 beta standard|10ng/tubex2
AR1107|Biotinylated anti- mouse IL-1 beta antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4