Mouse IFN-gamma ELISA Kit

Brand

Cell Sciences

Description

The Cell Sciences Mouse IFN-gamma ELISA (Enzyme-Linked Immunosorbent Assay) kit is an, in vitro, enzyme-linked immunosorbent assay for the quantitative measurement of mouse IFN-gamma in serum, plasma and cell culture supernatants. This assay employs an antibody specific for mouse IFN-gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and IFN-gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IFN-gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFN-gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Specificity

Mouse IFN-gamma

Sensitivity

< 5 pg/ml

Sample Types

Serum, plasma, and cell culture supernatants

spezie

Mouse

Cross Reaction

Mouse

detection target

Mouse IFN-gamma
Antigen: IFN-gamma

alias

IFG, IFI, interferon gamma, IFN-gamma, immune interferon

sensitivity

< 5 pg/ml

sample size

1

pack size

PP

UNSPSC

41116126 and E