Mouse G-CSF/CSF3 ELISA Kit

Brand

Cell Sciences

Description

G-CSF is an O-glycosylated glycoprotein, which is secreted by monocytes, macrophages and neutrophils. G-CSF is also produced by stromal cells, fibroblasts, and endothelial cells. Epithelial carcinomas, acute myeloid leukemia cells and various tumor cell lines, also express this factor. G-CSF stimulates the proliferation and differentiation of hematopoietic progenitor cells committed to the neutrophil/granulocyte lineage. It is a mitogen for some mouse myeloid leukemia cells and also for some carcinoma cell lines. In vitro G-CSF enhances the antibody-dependent cellular cytotoxicity of granulocytes against tumor cells.The Cell Sciences Mouse G-CSF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse G-CSF in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for mouse G-CSF coated on a 96-well plate. Standards and samples are pipetted into the wells and G-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse G-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of G-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

spezie

Mouse

Cross Reaction

Mouse

detection target

Mouse G-CSF/CSF3
Antigen: CSF3/G-CSF

alias

C17orf33, CSF3OS, GCSF, mgC45931, granulocyte colony-stimulating factor, filgrastim, lenograstim, pluripoietin

sample size

1

pack size

PP

UNSPSC

41116126 and E