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Boster
For quantitative detection of mouse CD200 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Mouse
There is no detectable cross-reactivity with other relevant proteins.
mouse CD200Protein name: OX-2 membrane glycoproteinGene Full Name: CD200 moleculeabbreviation: CD200Background: OX-2 membrane glycoprotein, also known as CD200 is a human protein encoded by the CD200 gene. This gene is mapped to 3q13.2. The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. It is suggested that CD200-CD200R signaling plays a central role in macrophage fusion and osteoclast formation. ID:O54901 ID:17470
antigen identified by monoclonal antibody MRC OX-2| CD_antigen=CD200| CD200 antigen| MOX1| MOX2| MRC OX-2 antigen| My033| OX-2 membrane glycoprotein
Nativ and recombinant mouse CD200
a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
NSO, Q31-G232
<10pg/ml
31.2pg/ml-2000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|341|762|1104|375|790|1263 Standard deviation|14.66|43.43|68.45|23.25|56.09|85.88 CV(%)|4.3|5.7|6.2|6.2|7.1|6.8
15-20
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s mouse CD200 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD200 has been precoated onto 96-well plates. Standards(NSO, Q31-G232) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD200 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD200 amount of sample captured in plate.
Aliquot 0.1ml per well of the 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml mouse CD200 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each mouse CD200 standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000 O.D.|0.089|0.166|0.226|0.351|0.698|1.055|1.674|2.138
Catalog number|Description|Quantity-|96-well plate precoated with anti- mouse CD200 antibody|1ST0000-10|Lyophilized recombinant mouse CD200 standard|10ng/tube�2AR1107|Biotinylated anti- mouse CD200 antibody|130µl(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)AR1106-1|Sample diluent buffer|30mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Pack size | 1x96 Wells |
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Brand | Boster |
Country of Manufacture | USA |
Sensitivity | <10pg/ml |
Specific Against | Mouse |
Antigen Name | OX-2 membrane glycoprotein (CD200) |
Detection Range | 31.2pg/ml-2000pg/ml |
Alias | antigen identified by monoclonal antibody MRC OX-2, CD_antigen, CD200 antigen, MOX1, MOX2, MRC OX-2 antigen, My033, OX-2 membrane glycoprotein |