Mouse BAFF PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of mouse BAFF in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Mouse

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Mouse BAFF
Protein name: Tumor necrosis factor ligand superfamily member 13B
Gene Full Name: tumor necrosis factor (ligand) superfamily, member 13b
abbreviation: TNFSF13B
Background: B-cell activating factor(BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFLSF13B gene.1,2 BAFF is a cytokine that belongs to the tumor necrosis factor(TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells.3All these ligands act as heterotrimers(i.e. three of the same molecule) interacting with heterotrimeric receptors4, although BAFF has been known to be active as either a hetero- or homotrimer.5 The standard used in this kit is recombinant mouse soluble BAFF(A127-L309) with molecular weight 23.2KDa.
ID:Q9WU72
ID:24099

alias

B lymphocyte stimulator| B-cell-activating factor| BAFF| BLyS| CD257| Dendritic cell-derived TNF-like molecule| soluble form| TALL-1| TN13B_HUMAN| TNF- and APOL-related leukocyte expressed ligand 1| TNFSF13B| Tumor necrosis factor ligand superfamily member 13b

Origin

Nativ and recombinant mouse BAFF

Antibodies

a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat

Immunogene

NSO, A127-L309

sensitivity

<10pg/ml

detection range

46.9pg/ml-3000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|139|312|1508|145|354|1397
Standard deviation|4.9|11.5|58.8|14.4|33.6|124.3
CV(%)|3.5|3.7|3.9|9.9|9.5|8.9

TMB(tetramethylbenzidine) incubation time

15-20

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s mouse BAFF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for BAFF has been precoated onto 96-well plates. Standards(NSO, A127-L309) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BAFF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse BAFF amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 3000pg/ml, 1500pg/ml, 750pg/ml, 375pg/ml, 188pg/ml, 94pg/ml, 46.9pg/ml mouse BAFF standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. We recommend that each mouse BAFF standard solution and each sample is measured in duplicate.

tipical result

Concentration(pg/ml)|0|46.9|94|188|375|750|1500|3000 O.D.|0.078|0.147|0.201|0.315|0.507|0.891|1.516|2.346

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- mouse BAFF antibody|1
ST0000-10|Lyophilized recombinant mouse BAFF standard|10ng/tubex2
AR1107|Biotinylated anti- mouse BAFF antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4