Mouse ANGPTL3 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of mouse ANGPTL3 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Mouse

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

mouse ANGPTL3
Protein name: Angiopoietin-related protein 3
Gene Full Name: angiopoietin-like 3
abbreviation: ANGPTL3
Background: ANGPTL3, also known as Angiopoietin-like 3 or HYPLl, is a protein that in humans is encoded by the ANGPTL3 gene. It is mapped to 1p31.3. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is expressed predominantly in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain, and the C-terminal fibrinogen (FBN)-like domain. ANGPTL3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. It can inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels.
ID:Q9R182
ID:30924

alias

ANG 5|ANG-5|ANG5|Angiopoietin 5|Angiopoietin like 3|Angiopoietin related protein 3|Angiopoietin-5|Angiopoietin-like protein 3|Angiopoietin-related protein 3|ANGL3_HUMAN|ANGPT5|ANGPTL3|ANL3|FHBL2|OTTHUMP00000010719|UNQ153/PRO179

Origin

Nativ and recombinant mouse ANGPTL3

Antibodies

a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat

Immunogene

sf21, S17-T455

sensitivity

<10pg/ml

detection range

156pg/ml-10000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|1.2|3.7|6.4|1.5|3.9|6.8
Standard deviation|0.07|0.233|0.39|0.123|0.261|0.442
CV(%)|5.8|6.3|6.1|8.2|6.7|6.5

TMB(tetramethylbenzidine) incubation time

25-30

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s mouse ANGPTL3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for ANGPTL3 has been precoated onto 96-well plates. Standards(sf21, S17-T455) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ANGPTL3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse ANGPTL3 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml mouse ANGPTL3 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each mouse ANGPTL3 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000 O.D.|0.006|0.064|0.125|0.259|0.511|0.936|1.623|2.194

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- mouse ANGPTL3 antibody|1
ST0000-10|Lyophilized recombinant mouse ANGPTL3 standard|10ng/tube�2
AR1107|Biotinylated anti- mouse ANGPTL3 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4