Mouse ALK-1/ACVRL1 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of mouse ALK-1 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Mouse

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

mouse ALK-1
Protein name: Serine/threonine-protein kinase receptor R3
Gene Full Name: activin A receptor like type 1
abbreviation: ACVRL1
Background: Serine/threonine-protein kinase receptor R3 is an enzyme that in humans is encoded by the ACVRL1 gene. It is also known as activin receptor-like kinase 1, or ALK1. This gene encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2.
ID:Q61288
ID:11482

alias

Activin A receptor | ACVRL1 | ACVRLK1 | ALK-1 | ALK1 | ALK 1 | HHT | HHT2 | ORW2 | SKR3 | TSR-I | TSRI | Q61288

Origin

Nativ and recombinant mouse ALK-1

Antibodies

a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat

Immunogene

NSO, D23-P119

sensitivity

<15pg/ml

detection range

46.9pg/ml-3000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|163|582|1507|189|654|1620
Standard deviation|10.27|31.43|108.5|13.04|47.74|123.12
CV(%)|6.3|5.4|7.2|6.9|7.3|7.6

TMB(tetramethylbenzidine) incubation time

25-30

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s mouse ALK-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for ALK-1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: D23-P119) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ALK-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse ALK-1 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 3000pg/ml, 1500pg/ml, 750pg/ml, 375pg/ml, 187.5pg/ml, 93.8pg/ml, 46.9pg/ml mouse ALK-1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatants, serum or plasma (heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. We recommend that each mouse ALK-1 standard solution and each sample is measured in duplicate.

tipical result

Concentration(pg/ml)|0|46.9|93.8|187.5|375|750|1500|3000 O.D.|0.032|0.060|0.138|0.239|0.538|1.045|1.895|2.280

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- mouse ALK-1 antibody|1
ST0000-10|Lyophilized recombinant mouse ALK-1 standard|10ng/tube�2
AR1107|Biotinylated anti- mouse ALK-1 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4