Mouse ACE PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of mouse ACE in cell culture supernates, serum and plasma(heparin).

spezie

Mouse

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Mouse ACE
Protein name: Angiotensin-converting enzyme
Gene Full Name: angiotensin I converting enzyme
abbreviation: ACE
Background: Angiotensin-converting enzyme(ACE), an exopeptidase, is a circulating enzyme that participates in the body's renin-angiotensin system(RAS), which mediates extracellular volume(i.e. that of the blood plasma, lymph and interstitial fluid), and arterial vasoconstriction. It is secreted by pulmonary and renal endothelial cells and catalyzes the conversion of decapeptide angiotensin I to octapeptide angiotensin II. Using a DNA marker at the growth hormone gene locus, which they characterized as 'extremely polymorphic' and which showed no recombination with ACE, ACE was mapped to 17q22-q24, consistent with the in situ hybridization mapping to 17q23. ACE, or kininase II, is a dipeptidyl carboxypeptidase that plays an important role in blood pressure regulation and electrolyte balance by hydrolyzing angiotensin I into angiotensin II, a potent vasopressor, and aldosterone-stimulating peptide. The enzyme is also able to inactivate bradykinin, a potent vasodilator.
ID:P09470
ID:11421

alias

ACE 1 | ACE T | Angiotensin converting enzyme | Angiotensin Converting Enzyme 1 | Angiotensinconverting enzyme | Angiotensin-converting enzyme | CD143 antigen | DCP 1 | DCP, DCP1 | Dipeptidyl carboxypeptidase 1 | Dipeptidyl carboxypeptidase I | Kininase II | MVCD3 | P12821 | Peptidyl dipeptidase A

Origin

Nativ and recombinant mouse ACE

Antibodies

a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat

Immunogene

NSO, L35-Q1264

sensitivity

<10pg/ml

detection range

93.8pg/ml-6000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|0.64|1.98|4.25|0.85|2.02|5.32
Standard deviation|0.029|0.085|0.179|0.062|0.141|0.367
CV(%)|4.6|4.3|4.2|7.3|7|6.9

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s mouse ACE ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for ACE has been precoated onto 96-well plates. Standards(NSO, L35-Q1264) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ACE is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse ACE amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 6000pg/ml, 3000pg/ml, 1500pg/ml, 750pg/ml, 375pg/ml, 187.5pg/ml, 93.8pg/ml mouse ACE standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. We recommend that each mouse ACE standard solution and each sample is measured in duplicate.

tipical result

Concentration(pg/ml)|0|93.8|187.5|375|750|1500|3000|6000 O.D.|0.084|0.128|0.178|0.253|0.444|0.658|1.511|2.327

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- mouse ACE antibody|1
ST0000-10|Lyophilized recombinant mouse ACE standard|10ng/tubex2
AR1107|Biotinylated anti- mouse ACE antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4