Low Molecular Weight Heparin (LMWH) ELISA for Plasma

Brand

Lifespan Technologies

short description

Heparin is a glycosaminoglycan with alternating uronic acid and aminoglycoside units. It is an anticoagulant used either in its native unfractionated form (UFH) MW ~16 kD or in various partially depolymerized forms (LMWH) of 4-8 kD. The heparin-ELISA product number K-2000 is a quantitative enzyme-linked assay designed for the in vitro measurement of low molecular weight heparin levels in plasma. This assay measures heparin directly using a heparin binding protein which has been conjugated to HRP. The heparin-ELISA is a competitive assay in which the colorimetric signal is inversely proportional to the amount of heparin present in the sample. Samples to be assayed are first mixed with the Detector-Enzyme Conjugate in wells of the heparin coated plate. Heparin in the sample competes with heparin bound to the plate for binding of the Detector-Enzyme Conjugate. The concentration of heparin in the sample is determined using a standard curve of known amounts of heparin spiked into normal plasma. For best results, the heparin used for the standard curve should match the type of heparin being assayed.
Pipettes (8-channel multipipettor recommended) Absorbance microplate reader Plate Cover Normal Plasma

spezie

Human

detection target

Low Molecular Weight Heparin (LMWH)

pack size

1x96 Wells

storage and Stability

Kit can be stored unopened at 4°C for up to six months. Opened solutions can be used for up to one week when stored at 4°C. The TMB solution should be protected from light. Reconstituted detector enzyme conjugate should be used immediately or aliquotted and stored at -80°C.

Kit Content

Heparin-coated 96-well plate Pipettes (8-channel multipipettor recommended) Conjugate Diluent Absorbance microplate reader Detector-Enzyme Conjugate Plate Cover TMB Solution Normal Plasma Stop Solution, 0.5M H2SO4 Wash Concentrate 10X, (dilute 1 part plus 9 parts purified H2O to make TBS-0.05% TWEEN 20)