Profile Informations
Login Datas
or login
Boster
For quantitative detection of human TNFRSF7 in cell culture supernates and serum.
Human
There is no detectable cross-reactivity with other relevant proteins.
Human TNFRSF7Protein name: CD27 antigenGene Full Name: CD27 moleculeabbreviation: CD27Background: CD27 antigen, also called CD27 or TNFRSF7 is a tumor necrosis factor receptor. This gene is mapped to 12p13.31. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein(SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. ID:P26842 ID:939
CD 27|Cd27|CD27 antigen|CD27 molecule|CD27_HUMAN|CD27L receptor|LPFS2|MGC20393|OTTHUMP00000238557|S152|T cell activation antigen CD27|T cell antivation antigen S152|T-cell activation antigen CD27|T14|TNFRSF 7|TNFRSF7|TNFSF7|Tp 55|Tp55|Tumor necrosis factor receptor superfamily member 7
Nativ and recombinant human TNFRSF7
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
NSO, T21-I192
<1pg/ml
62.5pg/ml-4000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|427|1423|2832|546|1253|2637 Standard deviation|23.1|48.4|130.3|36.6|53.9|145.1 CV(%)|5.4|3.4|4.6|6.7|4.3|5.5
15-20
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s human TNFRSF7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFRSF7 has been precoated onto 96-well plates. Standards(NSO, T21-I192) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNFRSF7 amount of sample captured in plate.
Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml human TNFRSF7 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates or serum to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each human TNFRSF7 standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000 O.D.|0.036|0.118|0.194|0.331|0.587|1.009|1.567|2.242
Catalog number|Description|Quantity-|96-well plate precoated with anti- human TNFRSF7 antibody|1ST0000-10|Lyophilized recombinant human TNFRSF7 standard|10ng/tubex2AR1107|Biotinylated anti- human TNFRSF7 antibody|130ul(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)AR1106-1|Sample diluent buffer|30mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Pack size | 1x96 Wells |
---|---|
Brand | Boster |
Country of Manufacture | USA |
Sensitivity | <1pg/ml |
Specific Against | Human |
Antigen Name | CD27 antigen (CD27) |
Detection Range | 62.5pg/ml-4000pg/ml |
Alias | CD 27, Cd27, CD27 antigen, CD27 molecule, CD27_HUMAN, CD27L receptor, LPFS2, MGC20393, OTTHUMP00000238557, S152, T cell activation antigen CD27, T cell antivation antigen S152, T-cell activation antigen CD27, T14, TNFRSF 7, TNFRSF7, TNFSF7, Tp 55, Tp55, T |