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Boster
For quantitative detection of human TNFRSF18 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Human
There is no detectable cross-reactivity with other relevant proteins.
Human TNFRSF18Protein name: Tumor necrosis factor receptor superfamily member 18Gene Full Name: tumor necrosis factor receptor superfamily, member 18abbreviation: TNFRSF18Background: Tumor necrosis factor receptor superfamily member 18(TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. This gene is mapped to 1p36.33. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. ID:Q9Y5U5 ID:8784
Activation inducible TNFR family receptor|AITR|GITR|GITR D|Glucocorticoid induced TNFR related protein|Tumor necrosis factor receptor superfamily member 18
Nativ and recombinant human TNFRSF18
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
NSO, Q26-E161
<10pg/ml
62.5pg/ml-4000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|342|1565|3014|540|1896|3242 Standard deviation|11.97|62.6|153.7|30.24|110|217.2 CV(%)|3.5|4|5.1|5.6|5.8|6.7
25-30
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s human TNFRSF18 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFRSF18 has been precoated onto 96-well plates. Standards(NSO, Q26-E161) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF18 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNFRSF18 amount of sample captured in plate.
Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml human TNFRSF18 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each human TNFRSF18 standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000 O.D.|0.031|0.095|0.175|0.299|0.596|1.008|1.651|2.111
Catalog number|Description|Quantity-|96-well plate precoated with anti- human TNFRSF18 antibody|1ST0000-10|Lyophilized recombinant human TNFRSF18 standard|10ng/tube�2AR1107|Biotinylated anti- human TNFRSF18 antibody|130µl(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)AR1106-1|Sample diluent buffer|30 mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Pack size | 1x96 Wells |
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Brand | Boster |
Country of Manufacture | USA |
Sensitivity | <10pg/ml |
Specific Against | Human |
Antigen Name | Tumor necrosis factor receptor superfamily member 18 (TNFRSF18) |
Detection Range | 62.5pg/ml-4000pg/ml |
Alias | Activation inducible TNFR family receptor, AITR, GITR, GITR D, Glucocorticoid induced TNFR related protein, Tumor necrosis factor receptor superfamily member 18 |