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Boster
For quantitative detection of human TNFRSF17 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Human
There is no detectable cross-reactivity with other relevant proteins.
Human TNFRSF17Protein name: Tumor necrosis factor receptor superfamily member 17Gene Full Name: tumor necrosis factor receptor superfamily, member 17abbreviation: TNFRSF17Background: TNFRSF17(Tumor necrosis factor receptor superfamily member 17), also called BCMA, is a protein that in humans is encoded by the TNFRSF17 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily and is mapped to 16p13.13. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor(ligand) superfamily, member 13b(TNFSF13B/TALL-1/BAFF), and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation. ID:Q02223 ID:608
B cell maturation antigen|B cell maturation factor|B cell maturation protein|B-cell maturation protein|BCM|BCMA|CD 269|CD269|CD269 antigen|TNFRSF 17|TNFRSF17|TNR17_HUMAN|Tumor necrosis factor receptor member 17|Tumor necrosis factor receptor superfamily member 17
Nativ and recombinant human TNFRSF17
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
NSO, M1-A54
<10pg/ml
31.2pg/ml-2000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|134|642|1286|158|614|1358 Standard deviation|4.69|35.95|61.73|6.64|41.14|73.33 CV(%)|3.5|5.6|4.8|4.2|6.7|5.4
25-30
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s human TNFRSF17 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFRSF17 has been precoated onto 96-well plates. Standards(NSO, M1-A54) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF17 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNFRSF17 amount of sample captured in plate.
Aliquot 0.1ml per well of the 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human TNFRSF17 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each human TNFRSF17 standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000 O.D.|0.007|0.081|0.171|0.360|0.654|1.150|1.793|2.109
Catalog number|Description|Quantity-|96-well plate precoated with anti- human TNFRSF17 antibody|1ST0000-10|Lyophilized recombinant human TNFRSF17 standard|10ng/tubex2AR1107|Biotinylated anti- human TNFRSF17 antibody|130ul(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)AR1106-1|Sample diluent buffer|30mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Pack size | 1x96 Wells |
---|---|
Brand | Boster |
Country of Manufacture | USA |
Sensitivity | <10pg/ml |
Specific Against | Human |
Antigen Name | Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) |
Detection Range | 31.2pg/ml-2000pg/ml |
Alias | B cell maturation antigen, B cell maturation factor, B cell maturation protein, B-cell maturation protein, BCM, BCMA, CD 269, CD269, CD269 antigen, TNFRSF 17, TNFRSF17, TNR17_HUMAN, Tumor necrosis factor receptor member 17, Tumor necrosis factor receptor |