Profile Informations
Login Datas
or login
Boster
For quantitative detection of human TNFRSF13C in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Human
There is no detectable cross-reactivity with other relevant proteins.
human TNFRSF13CProtein name: Tumor necrosis factor receptor superfamily member 13CGene Full Name: tumor necrosis factor receptor superfamily, member 13Cabbreviation: TNFRSF13CBackground: Tumor necrosis factor receptor superfamily member 13C also known as B-cell-activating factor receptor or BAFF receptor is a protein that in humans is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating factor(BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival. ID:Q96RJ3 ID:115650
B Cell Activating Factor Receptor|B-cell-activating factor receptor|BAFF R|BAFF receptor|BAFF-R|BAFFR|BLyS receptor 3|BlySR3|BR 3|BR3|BROMIX|CD 268|CD268|CD268 antigen|CVID4|MGC138235|OTTHUMP00000028746|Prolixin|TNFRSF 13C|TNFRSF13C|TR13C_HUMAN|Tumor Necrosis Factor Receptor Subunit Member 13C|Tumor necrosis factor receptor superfamily member 13C
Nativ and recombinant human TNFRSF13C
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
NSO, S7-A71
<10pg/ml
78pg/ml-5000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|643|1604|3262|745|1862|3657 Standard deviation|29.6|57.75|169.6|43.21|91.3|234.1 CV(%)|4.6|3.6|5.2|5.8|4.9|6.4
20-25
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s human TNFRSF13C ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFRSF13C has been precoated onto 96-well plates. Standards(NSO, S7-A71) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF13C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNFRSF13C amount of sample captured in plate.
Aliquot 0.1ml per well of 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 313pg/ml, 156pg/ml, 78pg/ml human TNFRSF13C standard solutions into the pre-coated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each human TNFRSF13C standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|78|156|312|625|1250|2500|5000 O.D.|0.003|0.137|0.248|0.486|0.777|1.260|1.704|1.925
Catalog number|Description|Quantity-|96-well plate precoated with anti- human TNFRSF13C antibody|1ST0000-10|Lyophilized recombinant human TNFRSF13C standard|10ng/tube�2AR1107|Biotinylated anti- human TNFRSF13C antibody|130µl(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)AR1106-1|Sample diluent buffer|30mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Pack size | 1x96 Wells |
---|---|
Brand | Boster |
Country of Manufacture | USA |
Sensitivity | <10pg/ml |
Specific Against | Human |
Antigen Name | Tumor necrosis factor receptor superfamily member 13C (TNFRSF13C) |
Detection Range | 78pg/ml-5000pg/ml |
Alias | B Cell Activating Factor Receptor, B-cell-activating factor receptor, BAFF R, BAFF receptor, BAFF-R, BAFFR, BLyS receptor 3, BlySR3, BR 3, BR3, BROMIX, CD 268, CD268, CD268 antigen, CVID4, MGC138235, OTTHUMP00000028746, Prolixin, TNFRSF 13C, TNFRSF13C, TR |