Human TNFRSF13B/TACI PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human TNFRSF13B in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human TNFRSF13B
Protein name: Tumor necrosis factor receptor superfamily member 13B
Gene Full Name: tumor necrosis factor receptor superfamily, member 13B
abbreviation: TNFRSF13B
Background: Tumor necrosis factor receptor superfamily member 13B, also known as TNFRSF13B or more commonly as TACI, is a transmembrane receptor protein found predominantly on the surface of B cells, which are an important part of the immune system. TACI controls T cell-independent B cell antibody responses, isotype switching, and B cell homeostasis. TACI is a lymphocyte-specific member of the tumor necrosis factor(TNF) receptor superfamily. It was originally discovered because of its ability to interact with calcium-modulator and cyclophilin ligand(CAML). TACI was later found to play a crucial role in humoral immunity by interacting with two members of the TNF family: BAFF and APRIL. These proteins signal through TACI inducing activation of several transcription factors including NFAT, AP-1, and NF-kappa-B which then modulate cellular activities. Defects in the function of TACI can lead to immune system diseases.
ID:O14836
ID:23495

alias

CD267|TNFRSF13B|TR13B_HUMAN|Transmembrane activator and CAML interactor|Tumor necrosis factor receptor superfamily member 13B

Origin

Nativ and recombinant human TNFRSF13B

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, S2-T166

sensitivity

<10pg/ml

detection range

93.7pg/ml-6000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|527|2085|3561|686|2664|3724
Standard deviation|19|91.74|185.2|31.6|151.8|242.1
CV(%)|3.6|4.4|5.2|4.6|5.7|6.5

TMB(tetramethylbenzidine) incubation time

15-20

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human TNFRSF13B ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFRSF13B has been precoated onto 96-well plates. Standards(NSO, S2-T166) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF13B is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNFRSF13B amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 6000pg/ml, 3000pg/ml, 1500pg/ml, 750pg/ml, 375pg/ml, 187.5pg/ml, 93.7pg/m human TNFRSF13B standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human TNFRSF13B standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|93.7|187.5|375|750|1500|3000|6000 O.D.|0.036|0.112|0.160|0.302|0.514|0.977|1.686|2.343

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human TNFRSF13B antibody|1
ST0000-10|Lyophilized recombinant human TNFRSF13B standard|10ng/tube�2
AR1107|Biotinylated anti- human TNFRSF13B antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4